Journal:

Article Title: Time of Day differences in the Number of Cytokine-, Neurotrophin- and NeuN-immunoreactive cells in the Rat Somatosensory or Visual Cortex

doi: 10.1016/j.brainres.2010.04.012

Figure Lengend Snippet: Quantitative evaluation of the number of TNF-, IL1β, NGF-, NeuN- and Fos-IR cells in the Sctx and Vctx layers at the end of the light (1100) or dark (2300) period. Data are represented as Mean ± S.E.M.

Article Snippet: Immunohistochemistry was performed as described previously ( Churchill et al., 2005 , 2008 ), using a polyclonal anti-human Fos antibody produced in rabbit (1:10,000; Calbiochem (Oncogene Research Products), San Diego, CA), goat anti-rat IL1β (0.5 μg/ml, R&D Systems, Inc., Minneapolis, MN), goat anti-rat TNFα (0.5 μg/ml, R&D Systems, Inc., Minneapolis, MN), rabbit anti-mouse NGF that cross reacts with rat NGF (1:2500 or 1:5000; Chemicon, Temecula, CA) or mouse monoclonal antibody to NeuN (1:500; Chemicon, Temecula, CA).

Techniques:

Journal:

Article Title: Time of Day differences in the Number of Cytokine-, Neurotrophin- and NeuN-immunoreactive cells in the Rat Somatosensory or Visual Cortex

doi: 10.1016/j.brainres.2010.04.012

Figure Lengend Snippet: IL1β-IR cells increased in Sctx layers II–V at 2300 h (C, D & F) in comparison to 1100 h (A, B, E). The IL1β-IR appears in the cytoplasm and extends into the apical dendrite of the large pyramidal neurons in layer V at 2300 h (D & F). Bar = 0.05 mm for A–D; Bar =0.025 mm for E&F.

Article Snippet: Immunohistochemistry was performed as described previously ( Churchill et al., 2005 , 2008 ), using a polyclonal anti-human Fos antibody produced in rabbit (1:10,000; Calbiochem (Oncogene Research Products), San Diego, CA), goat anti-rat IL1β (0.5 μg/ml, R&D Systems, Inc., Minneapolis, MN), goat anti-rat TNFα (0.5 μg/ml, R&D Systems, Inc., Minneapolis, MN), rabbit anti-mouse NGF that cross reacts with rat NGF (1:2500 or 1:5000; Chemicon, Temecula, CA) or mouse monoclonal antibody to NeuN (1:500; Chemicon, Temecula, CA).

Techniques: Comparison

Journal:

Article Title: Time of Day differences in the Number of Cytokine-, Neurotrophin- and NeuN-immunoreactive cells in the Rat Somatosensory or Visual Cortex

doi: 10.1016/j.brainres.2010.04.012

Figure Lengend Snippet: IL1β-IR cells increased in Vctx layers V–VI at 2300 h (D & F) in comparison to 1100 h (B, E). The IL1β-IR appears in the cytoplasm and extends into the apical dendrite of the large pyramidal neurons in layer V at 2300 h (D & F). Bar = 0.05 mm for A–D; Bar =0.025 mm for E&F.

Article Snippet: Immunohistochemistry was performed as described previously ( Churchill et al., 2005 , 2008 ), using a polyclonal anti-human Fos antibody produced in rabbit (1:10,000; Calbiochem (Oncogene Research Products), San Diego, CA), goat anti-rat IL1β (0.5 μg/ml, R&D Systems, Inc., Minneapolis, MN), goat anti-rat TNFα (0.5 μg/ml, R&D Systems, Inc., Minneapolis, MN), rabbit anti-mouse NGF that cross reacts with rat NGF (1:2500 or 1:5000; Chemicon, Temecula, CA) or mouse monoclonal antibody to NeuN (1:500; Chemicon, Temecula, CA).

Techniques: Comparison

Journal:

Article Title: Time of Day differences in the Number of Cytokine-, Neurotrophin- and NeuN-immunoreactive cells in the Rat Somatosensory or Visual Cortex

doi: 10.1016/j.brainres.2010.04.012

Figure Lengend Snippet: IL1β-IR astrocytes increased in number at 2300 h in layer VI (B& D) and the white matter below this layer (external capsule for the Sctx and forceps major (fmj) relative to 1100 h (A&C). The Vctx astrocytes (A&B) were much darker than the Sctx astrocytes (C & D). The layer VI and fibrous astrocytes in the white matter are marked by the arrows. Bar = 0.05 mm.

Article Snippet: Immunohistochemistry was performed as described previously ( Churchill et al., 2005 , 2008 ), using a polyclonal anti-human Fos antibody produced in rabbit (1:10,000; Calbiochem (Oncogene Research Products), San Diego, CA), goat anti-rat IL1β (0.5 μg/ml, R&D Systems, Inc., Minneapolis, MN), goat anti-rat TNFα (0.5 μg/ml, R&D Systems, Inc., Minneapolis, MN), rabbit anti-mouse NGF that cross reacts with rat NGF (1:2500 or 1:5000; Chemicon, Temecula, CA) or mouse monoclonal antibody to NeuN (1:500; Chemicon, Temecula, CA).

Techniques:

Journal:

Article Title: Time of Day differences in the Number of Cytokine-, Neurotrophin- and NeuN-immunoreactive cells in the Rat Somatosensory or Visual Cortex

doi: 10.1016/j.brainres.2010.04.012

Figure Lengend Snippet: Quantitative evaluation of the number of IL1β-IR astrocytes in the Sctx and Vctx layers at the end of the light (1100) or dark (2300) period. Data are represented as Mean ± S.E.M. for n= 7 rats.

Article Snippet: Immunohistochemistry was performed as described previously ( Churchill et al., 2005 , 2008 ), using a polyclonal anti-human Fos antibody produced in rabbit (1:10,000; Calbiochem (Oncogene Research Products), San Diego, CA), goat anti-rat IL1β (0.5 μg/ml, R&D Systems, Inc., Minneapolis, MN), goat anti-rat TNFα (0.5 μg/ml, R&D Systems, Inc., Minneapolis, MN), rabbit anti-mouse NGF that cross reacts with rat NGF (1:2500 or 1:5000; Chemicon, Temecula, CA) or mouse monoclonal antibody to NeuN (1:500; Chemicon, Temecula, CA).

Techniques: